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Successfully complete the game under the normal or hard difficulty setting.

After defeating Dorothy in your first fight, you will go to the start of the level and make your way past the rooms. You will fight a boss named Spider. Try to move away from the dangling spiders. His weakness is Fire (red serum). When a spider gets on you press [R1] (shield) to get them off. You cannot attack him with D-Felon (blue serum). When he comes down from his web, use Fire or Nalcon on him. After you defeat him, you obtain a Skip (allows you to improve a serum ability). Use your Skip on the Nalcon, as it will be needed later. Then, open the gate and cross the bridge to Dorothy.

In the Enriched Uranium Refinery, in the room where you format the security card to "Saturnus", defeat all the red and gray robots after you talk to Ash over the security screen.

Successfully complete the game under the normal difficulty setting with a "B" or higher rank.

Successfully complete the game under the hard difficulty setting with a "S" rank.

Successfully complete the game under the easy or normal difficulty setting. Load the cleared saved game to toggle short mode.